WebThermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also …
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WebAug 31, 2024 · The reaction system was prepared using the EasyTaq® DNA Polymerase kit (TransGen Biotech, Beijing, China): 1 μl EasyTaq® DNA Polymerase, 5 μl template, 2 μl 10× EasyTaq® Buffer, 2 μl 2.5 mM dNTPs, 2 μl primer mix, 8 μl ddH 2 O. Each 100 μl of primer mix contains 1 μl of primers of all the 33 targets bacterium, IC, and human DNA ... WebSep 16, 2024 · PCR reaction system (25 μL) included 2.5 μL of 10 × EasyTaq ® Buffer, 2 μL of 2.5 mM dNTPs, 0.5 μL of 5 U/μL EasyTaq DNA Polymerase, 0.5 μL of 10 μM each primer pair of eight species, DNA mixture of eight species at the indicated concentration and refilled ddH 2 O to 25 μL. PCR amplification was performed by T100™ Thermal Cycler ...
WebJun 23, 2024 · PCR system was composed of 2.5 μl of 10 x EasyTaq ® Buffer, 2 μl of 2.5 mM dNTPs, 0.5 μl of EasyTaq DNA polymerase, 0.5 μl of 10 μm each primer, genomic DNA of each species, and refilled ddH 2 O to 25 μl in a single reaction. The reaction was elicited by a 5-min denaturation at 94°C, followed by 34 cycles (94°C for 30 s, 63°C for 30 s ... WebEasyTaq® PCR SuperMix is a ready-to-use mixture of EasyTaq® DNA Polymerase, dNTPs, and optimized reaction buffer at a concentration of 2×. 2×EasyTaq® PCR …
WebOct 1, 2016 · EasyTaq DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. This exceptionally performant Taq DNA … WebNov 16, 2024 · We performed the PCR experiment using 20 μl reaction mixtures containing 100 ng genomic DNA, 2 μl 10× EasyTaq Buffer (with Mg 2+), 1.5 μl 2.5 mM dNTPs, 2 μl each of 2 μM primer stock, and 1 U EasyTaq DNA Polymerase (TransGen Biotech, Beijing, China) under the following thermal cycler conditions: 95°C for 5 min, then 35 cycles at …
WebDec 15, 2024 · downstream primers, 1 µl of EasyTaq® DNA polymerase, and 20 µl of P-mother liquor (P-mother liquor was prepared by mixing the following reagents: 10× EasyTaq® buffer:2.5 mM dNTPs:H 2O=5:3:32). The reaction procedure was as follows: 94 °C for 3 min, 35 cycles of 94 °C for 30 s, 60 °C for 30 s, and 72 °C
Web10×EasyTaq® Buffer 5 μl 1× 2.5 mM dNTPs 4 μl 0.2 mM EasyTaq® DNA Polymerase 0.5-1 μl 2.5-5 units Nuclease-free Water Variable - Total volume 50 μl - Reaction … medullary carcinoma thyroid amyloidWeb2,点样：在PCR后的DNA产物加入loading buffer以对DNA进行沉淀和染色，20μl的DNA中加入2μl的10*loading buffer或4μl的6*loading buffer。 点样时注意逐个点样，不要点错，最好把中间的那个孔留着点Marker，这样可避免点样的出错。Marker的选择依基因片段大小而定。 name brand bow tiesWebA 2 μl suspension was used as template in PCR reactions. PCR reactions were performed in a 20 μl volume containing 2 μl 10 × EasyTaq buffer, 200 μM dNTPs, 0.2 μM of each primer, and 1 U EasyTaq DNA polymerase (TransGen Biotech, Beijing, China). The primers used in this study were listed in Table 1. The PCR procedures were: 95 °C for 3 ... name brand cat foodWebEasyTaq® DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous … medullary cavity deutschWebTaKaRa Taq DNA Polymerase is a recombinant-version Taq polymerase derived from the Thermus aquaticus YT-1 strain, and is suitable for routine PCR applications.. For individual reaction setup and optimization, use individual components of enzyme, dNTP mixture, and 10X reaction buffer (with or without Mg 2+).A convenient 2X PCR master mix of Takara … medullary ca thyroidWebThe SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H 2 O. • TransTaq ® High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq ® DNA Polymerase. • Extension rate is about 1-2 kb/min. • Template-independent "A" can be generated at the 3' end of the ... medullary carcinoma thyroid herWebEasyTaq® DNA Polymerase The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq DNA Polymerase has 5'-->3' DNA polymerase … medullary cavity contains