WebNov 1, 2016 · Thus, a genomic test platform has been developed, denoted the Genomic Allergen Rapid Detection (GARD) assay. Due to the high informational content of the GARD test, accurate predictions of both the skin and respiratory sensitizing capacity of chemicals, have been demonstrated. Based on a matured dendritic cell line, acting as a human-like ... WebJun 19, 2024 · Genomic allergen rapid detection (GARD) is an in vitro assay for the prediction of skin sensitizing capacity of chemicals. The GARD assay monitors changes …
Is a combination of assays really needed for non-animal ... - PubMed
WebJun 11, 2024 · TG adaption [60], such as the Genomic Allergen Rapid Detection (GARD) assay [63,64], which is based on the measurements of a biomarker signature of genes associat ed with . WebJun 30, 2024 · In the IL-8 Luc assay, the changes in IL-8 expression are measured indirectly via the activity of a luciferase gene under the control of the IL-8 promoter. The relative … change network 2 name
The use of Genomic Allergen Rapid Detection (GARD) assays
WebApr 1, 2013 · The assay is based on the measurement of these transcripts, collectively termed the GARD Prediction Signature, using a complete genome expression array. … WebThe predictive performance of a single assay, the Genomic Allergen Rapid Detection (GARD™) assay, was compared with the performance, singly and in combination, of three formally validated non-animal approaches that appear as OECD test guidelines: the direct peptide reactivity assay (DPRA), the ARE-Nrf2 luciferase test method, and the human ... WebJul 6, 2024 · The IL-8 Luc Assay measures luciferase gene induction as an indicator of IL-8 activity in a THP-1-derived IL-8 reporter cell line. A fourth assay, the Genomic Allergen Rapid Detection (GARD) assay, uses a human myeloid cell line derived from MUTZ-3 to predict sensitising potential and potency based on gene expression profiling and is … hardware havelock nc