Psicheck-2 promega
WebpsiCHECK™-2 Vector 20μg C8021 Storage Conditions:Store the psiCHECK™-1 and psiCHECK™-2 Vectors at –20°C. Promega Corporation ·2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526· Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TB329 Printed in USA. Page 2 Revised 6/09
Psicheck-2 promega
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http://www.honorgene.com/uploads/files/202404/5ea518d17dd8f.pdf WebAug 13, 2024 · The 3′UTR of CREBL2 and PCBP2 were sub-cloned into the psiCHECK-2 vector (Promega, USA) downstream of the Renilla luciferase reporter gene. Flanking sequence on each side was synthetized with a short extension containing cleavage sites for XhoI (CTCGAG) and NotI (GCGGCCGC), and the primer sequences are shown in Table …
WebpsiCHECK-2 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available … WebDual-luciferase reporter assay The psiCHECK-2 vector ( Promega, Cat # C8021) was used to construct plasmids for dual-luciferase reporter assay. RNF146 3′UTR or its mutant was cloned October 12, 2024 Zhaowei Wang, Xiaoling Xia, Jiaqi Li, Tatsushi Igaki
WebpsiCHECK-2 (Search Vector Database) Backbone manufacturer Promega Backbone size w/o insert (bp) 6273 Total vector size (bp) 6302 Vector type Mammalian Expression, Luciferase ; microRNA activity Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert WebFeb 15, 2024 · The PCR products for the wild-type and mutant target genes were cloned into multiple cloning regions of the vector psiCHECK-2 (Promega, Madison, WI, USA) using the NotI and XhoI restriction sites ...
WebHere we demonstrate the utility of the psiCHECK™-2 Vector, originally designed for screening siRNA silencing efficiency, as a biosensor for microRNA activity in cells. Promega Notes 99, 16–18. Article MicroRNA-Biosensors-Application-for-the-psiCHECK-2-Vector PDF (184 KB) – English Related Products psiCHECK™-2 Vector Related Protocols
WebThe psiCheck-2 vector contains two luciferase genes, renilla as the experimental reporter while firefly the control reporter. Renilla is the experimental reporter as you can clone in … diapers rash home remedy bathtubWebFrom the psiCHECK-2 vector (Promega, Cat. No. C8021), a 231-nucleotide (nt) region was selected from the firefly luciferase gene sequence available for (5) Tumor elimination by … citibike online loginWebThe psiCHECK™-2 Vector contains a second reporter gene, firefly luciferase, and is designed for endpoint lytic assays. Introduction of firefly luciferase in the psiCHECK™-2 Vector … diapers remix key glockWebpsiCHECK2 (Search Vector Database) Backbone manufacturer Promega Backbone size (bp) 6273 Modifications to backbone Insertion of a SalI/NotI/XhoI linker between the NotI and XhoI site Vector type Mammalian Expression Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number diapers return policyWebThe psiCHECK™-1 Vector and psiCHECK™-2 Vector provide a quantitative and rapid approach to optimize RNA interference (RNAi). The vectors enable monitoring of changes in expression of a target gene fused to the reporter gene. citi bike nyc discountWebMar 8, 2024 · The RNAi target sequence was cloned into the 3′ UTR of the Renilla luciferase gene on a psiCHECK-2 (Promega) vector, and firefly luciferase was used as a reference control. Cells were incubated and transfected in 48-well cluster plates. Cells were seeded 1 day prior to transfection and transfected at 50% confluency. citi bike price increaseWebThe psiCHECK™-2 Vector is designed to provide quantitative and rapid optimization of RNA interference (RNAi). The vector enables monitoring of changes in expression of a target … diapers shapewear and breast forms